Bloody Minded

“It’s pronounced nuc-ul-ophosmin” “Nucleophosmin”. “Noo-clee-o-phosmin”. FLT3-ITD got you In The Dumps? Have a Total Knowledge Deficit about TKD mutations? Struggling with the A-B-Cs of NPM1 mutations? Want to know how a cat sarcoma is somehow related to AML? Join the Bloody Minded Crew as they flit about the laboratory looking at the structure of FLT3 and NPM1, what these proteins usually do and how we test for mutations in the advanced myeloid episode.
Timestamps
0:52 - Bloody Minded issues a formal apology
2:40 - Introducing molecular chaperones
5:05 - What even NPM1?
9:30 - Functions of NPM1
10:55 - Molecular biology of NPM1 in disease
15:42 - Why the subtypes are important
19:53 - NPM1 prognosis
21:35 - MRD testing in NPM1
25:52 - NPM1 summary
27:13 - Intro to FLT3
28:30 - Mandatory FLT3 history
36:28 - FLT3 pathogenesis
37:37 - FLT3 structure, ITD vs TKD
41:16 - The juxtamembrane domain
42:53 - Prognostic features in FLT3
44:17 - TKD mutations
46:26 - FLT3 in the lab
50:30 - Take home messages
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Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au

U-G-L-Y, you ain't got no alibi, you dysplastic yeah yeah you dysplastic. Apologies to all the ring sideroblasts out there, but this week the Bloody Minded crew is doing a Mean Girls and judging cells by their morphology. For now we’re deciding to ignore those that would argue that it’s what’s on the inside that counts (i.e. judging by NGS… that’ll come later).
TIMESTAMPS
2:28 - For some reason, an audio podcast on visual dysplasia 
5:05 - Normal erythroid morphology 
7:17 - Dysplastic erythroid morphology 
17:32 - Normal granulocyte morphology 
20:00 - Dysplastic granulocyte morphology 
28:33 - Monocyte morphology - dysplastic at baseline 
30:33 - Normal megakaryocyte morphology 
32:24 - Dysplastic megakaryocyte morphology 
35:02 - Take Home Messages
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Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au

What do arsenic, acne tablets and Jodi Picoult have in common? Why, it must be a deep dive with Nick, Bashar and Cale on acute promyelocytic leukaemia. From buzzwords to brain bleeds, the sweetest of translocations to the cruellest of coagulopathies... The yield is almost as high as the survival rates.
TIMESTAMPS
00:57 - Bloody Minded Poetry Jam & Jodi Picoult
06:25 - APML Starts Here
08:02 - APML - Where does it fit in AML?
10:02 - Morphology & Flow
12:12 - PML::RARA - t(15;17) and rare variants
16:58 - Lab Summary
17:50 - Clinical Presentation
21:02 - APML Coagulopathy
26:46 - Treatment and Risk Stratification
33:53 - High Risk Aside
36:58 - Differentiation Syndrome
41:04 - Treatment SEs
44:18 - Microgranular Variant
47:00 - Summary and Takehomes
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Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au

After our initial intro to AML back in ep 79, we are back to dig deeper into AML this week, from pathophysiology through to treatment overview. The boys are joined by final year registrar Dr Georgina Calov who is here to cut through our nonsense and deliver some actual high yield content. And thank goodness for that, otherwise it'd be an hour of us making bad April Fool's day jokes.
Timestamps
2:09 - Introducing Dr Georgina Calov
3:34 - AML Refresher
7:12 - AML defined by recurrent genetic abnormalities
15:39 - AML defined by differentiation (aka whats with the M numbers)
18:14 - MN-pCT
19:03 - Prognostication - ELN Classification
21:58 - Diagnosing AML - Clinical
24:16 - Diagnosing AML - Lab
31:39 - New AML Workup
35:50 - Intro to Treatment - Induction in "Fit" patients
41:40 - Treatment - Consolidation & MRD
45:15 - Treatment - "Unfit"
48:02 - Treatment - Supportive
50:16 - Treatment - Relapse/Refractory
55:19 - Summary
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Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au

Cat got your tongue? Or in this case - mucositis? Does converting the acute allogeneic transplant patient who can no longer eat's medications to IV fill you with dread? In this practical for once episode join consultant haematologist Dr Alison Chandler as well as haematology pharmacists Andriana Colic & Ariana McCauley as they take us through the how to of this challenging, but very common, clinical scenario. 
Timestamps
0:58 - Nick's literal nightmare
2:18 - Today's outline
3:38 - Let's meet the A-team!
11:14 - What do you wish the haem doctors knew?
13:38 - Introducing the NBM transplant patient
15:18 - Tacrolimus
17:37 - Mycophenolate
19:51 - Ciclosporine (or cyclosporine or CsA or CyA, whatevs)
22:20 - Hot tip on TDM
23:47 - Antifungals
24:23 - Posaconazole
26:06 - Voriconazole
27:29 - Isavuconazole
28:45 - Echinocandins - mica/anidula
30:52 - Antivirals
33:27 - CMV treatment
37:41 - Antibacterials - PJP prophylaxis
39:22 - The NBM APML patient 
43:35 - Antiepileptics
46:24 - TLS prophylaxis
47:53 - Wrap up time!
50:18 - Episode summary
Support the show
Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au