Bloody Minded

If we’re going to truly understand myeloid disease, we must dispense with the ward and journey to the dystopian heart that beats at the centre of each dysplastic cell. Learn what is valid and in-valid as we swim across an ocean of concepts - past the basic principles of DNA and RNA, Polymerase Chain Reaction and its many many variants, Fragment Analysis and Next Generation Sequencing. Just save a little something for the swim back to clinic next week.
PS The Extra T is for TYOTT 
Timestamps
0:55 - Back in the lab, baby!
2:08 - Today's outline
6:24 - What IS molecular testing?
8:13 - The central dogma of molecular biology
14:46 - Types of genetic mutations
20:05 - Nomenclature for gene variants (advanced)
23:52 - Samples for testing
27:42 - What comes after DNA extraction
30:11 - What is PCR and how did we discover it? 
38:45 - What we do post PCR
41:27 - Quantitative and RT-PCR 
46:54 - Asking the RIGHT question
48:55 - Digital droplet PCR
50:44 - Electrophoresis
52:35 - Fragment analysis and melt curve analysis
54:33 - NGS
1:00:02 - What is a VAF?
1:05:23 - Lab governance (for haem ATs)
1:09:32 - Take home messages
Support the show
Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au

Happy marrows are all alike; every unhappy marrow is unhappy in its own way.  When haematopoiesis behaves itself, it’s frankly quite boring. When it goes rogue, it can get very creative. Join Cale, Bash and Nick as they attempt to walk through how this process goes off the rails, how MDS and AML are best understood and how dysplasia, cytopenias, blasts and clones all fit together.
TIMESTAMPS
0:55 - Our serious introduction to counterbalance last week 
1:58 - Episode overview 
2:50 - Introducing MDS 
7:12 - Risk factors for MDS 
8:53 - MDS progression 
11:13 - Complications of MDS 
13:10 - Leukaemic transformation 
15:37 - MDS epidemiology 
16:27 - Diagnosing MDS 
21:19 - What is dysplasia? 
26:44 - What we test on marrow 
35:13 - Clonality assessment in MDS 
42:29 - Caveats in diagnosing MDS 
43:18 - MDS summary (finally) 
44:09 - MDS treatment in brief 
45:37 - Intro to AML 
51:32 - Episode summary 
Support the show
Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au

What’s a CHIP and why is it so delicious? Why do we still abbreviate myeodysplastic neoplasm to MDS? How did Bashar get his hands on the Necronomicon? These are questions that every budding haematologist asks themselves at some point and we will answer at least some of them here! Join us today for a birds-eye overview of the myeloid family of disorders before we dig into them more over the coming weeks. 
TIMESTAMPS
0:57 - Does anyone know a good priest?  
3:17 - Myeloid conditions in bucket form  
5:30 - Acute vs chronic in haematological diseases  
7:35 - Myeloid precursor lesions  
10:33 - Myeloproliferative neoplasms  
15:23 - Myelodysplastic syndromes  
18:50 - MDS/MPN Overlap Syndromes  
22:29 - Mastocytosis  
26:02 - Acute myeloid leukaemia  
28:23 - (Acute promyelocytic leukaemia)  
29:36 - Histiocytic/dendritic cell disorders  
31:14 - Take Home Messages
Support the show
Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au

Hi-ho, Hi-ho, it's off the myeloid factory we go. Grab your fastest belt, a spare inserter and all the iron plates you can get your hands on as the Bloody Minded Crew embark on their nerdiest references yet and learn all about the basic science behind myelopoiesis. You’d better hop on a train, because the only main bus here goes straight from the marrow out to the periphery carrying all sorts of myeloid cells. And some blue circuits. 
0:52 - New year same us
2:44 - Season overview: myeloid time, baby!
5:15 - What is the myeloid compartment?
7:29 - Myelopoiesis
12:49 - Stem cell destiny... does free will exist?
14:28 - The myeloid progenitor
16:30 - Red cells and platelets
17:46 - White cell maturation
19:34 - Hormonal control of myelopoiesis
21:25 - Historical diversion into GCSF
23:19 - Clinical use of stimulating factors
26:39 - GCSF Bone Pain
28:15 - Emergency Myelopoiesis
30:08 - Wrap Up
Support the show
Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au

So you’ve been asked to start a DOAC? How is that different from a NOAC? Is there a difference? Which one should you start? Is warfarin a WOAC? How do I initiate these drugs? Why is the dose weird at the beginning? Do I need to check my dose is appropriate? 
If oral anticoagulation has you discombobulated you are in the right place!! In part II on the practical management of anticoagulation we are here to clarify all your questions on the most common types of oral anticoagulation you will come across as a junior doctor on the wards.
Timestamps
1:44 - Part I Recap and Part II Introduction
3:44 - History of Warfarin
8:37 - Mechanism of Warfarin
13:01 - Monitoring Warfarin
16:52 - Why Warfarin? and warfarin tips
21:40 - Warfarin reversal
31:05 - Warfarin Skin Necrosis & Drug Interactions
36:08 - Warfarin in Special Circumstances
40:25 - DOACs Intro
41:12 - Dabigatran
48:25 - Anti-Xa Agents (Rivaroxaban / Apixaban)
52:38 - Anti-Xa Monitoring (or not)
59:28 - DOACS - Special Circumstances
1:05:58 - Wrapup

Support the show
Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au